Model：Tissuelyser-24
Brand：Shanghai Jingxin?
Specifications ：24 bits
Introduction：Shanghai Jingxin? Tissuelyser? tissue grinder is a special, fast, efficient, multi-tube consistent system. It can extract and purify the original DNA, RNA and protein from any source.
Shanghai Jingxin? Tissuelyser? tissue grinder (patented product, counterfeiting must be punished! 201620363493X) is a special, fast, efficient, multi-tube consistent system. It can extract and purify the original DNA, RNA and protein from any source (including soil, plant and animal tissues/organs, bacteria, yeast, fungi, spores, paleontological specimens, etc.).
This machine is also known as multi-sample tissue grinder, rapid tissue grinder, multi-sample tissue homogenizer, and rapid sample homogenization system。
Technical features：
Compared with other existing sample preparation methods, the Tissuelyser? system has the advantages of wide versatility, high efficiency and flexibility. The system avoids the laborious, time-consuming, low-efficiency and many other shortcomings of traditional methods such as grinding, homogenization, and ultrasonic treatment, and can efficiently, quickly and stably lyse and purify nucleic acids and proteins of various types of samples.
Features：
The Tissuelyser? series grinder adopts a special vertical up and down integrated vibration mode, through the high frequency reciprocating vibration, impact and shearing of the grinding beads (zirconia, steel balls, glass beads, ceramic beads). Achieve goals quickly. Make the ground samples more sufficient, more uniform, better sample repeatability, and no cross-contamination between samples
1. The number of operations is large and the effect is good
Efficient and fast work can complete the grinding of 2×24, 2×48, 4×96 samples in 1 minute. Save time and effort, with small differences between batches and within batches. The extracted protein has a higher specific activity and a longer nucleic acid fragment
2. No cross contamination
The sample tube is in a fully enclosed state during the crushing process, and disposable centrifuge tubes and beads can be used. The sample is kept in the tube completely to avoid cross-contamination between samples and external contamination
3. Easy to operate
3.1 The built-in program controller can set parameters such as grinding time and rotor vibration frequency;
3.2 Humanized operation interface
4. Good stability
4.1 The vertical oscillation method is adopted for more sufficient grinding and better stability;
4.2 During the operation of the instrument, the noise is less than 55dB, which will not interfere with other experiments or instruments.
5. Convenient for low temperature operation
When a low-temperature grinding environment is required, the adapter with the sample can be immersed in liquid nitrogen to cool for 1-2 minutes, and then moved to the host to quickly fix it to start grinding. There is no need to freeze again, saving liquid nitrogen.
6. Good repeatability
The same procedure is set for the same tissue sample to obtain the same grinding effect. Short working time, sample temperature will not ris
Grinding comparison：
Grindable samples:
1. Plant tissue: roots, stems, leaves, flowers, fruits, seeds, etc.
2. Animal tissue: brain, heart, lung, stomach, liver, thymus, kidney, intestine, lymph node, muscle, bone, etc.
3. Fungal bacteria: yeast, E. coli, etc.
4. Food and medicine: all kinds of food, tablets, etc.
5. Volatile samples: coal, oil shale, wax products, etc.
6. Plastics, polymers: PE, PS, textiles, resins, etc.
Operating procedures：
Technical Parameter:
The main parameters | Parameter range |
Application | Tissue homogenization, grinding, cell disruption, homogenization, material dispersion, preparation, sample mixing, shaking |
Sample processing | It can handle 24 samples at the same time in 15 seconds, including liquid nitrogen refrigeration adapters that can be used for 12 and 24 positions |
Compatible sample volume | Compatible sample book: 24*(0.2-0.5ML) /24*2ML /6*(7-15)ML /2*25ML /2*50ML, various specifications of grinding tubes can be customized |
Touch screen display | LCD screen display can be convenient and intuitive operation, and can be upgraded to touch screen display operation. |
Anti-vibration principle | The German innovative schleifen-1 working method, innovative anti-shock principle, and the vertical up and down movement, and the innovative grinding bead movement, ensure the idealization of sample processing and instant crushing effect. |
Feed size | No requirement, adjust according to adapter |
Discharge size | ~5μm |
The number of grinding platforms (the number of grinding tanks that can be accepted) | >2 |
Fastening device with automatic center positioning | Yes |
Homogenization speed | 0—70 HZ/sec, working time: 0 second to 9999 minutes, users can set by themselves; |
Diameter of grinding ball: | 0.1-30mm |
Grinding ball material: | Alloy steel, chromium steel, zirconia, tungsten carbide, quartz sand; |
Accelerate | Reach the ideal speed in 2 seconds |
Decelerate | Reach the ideal speed in 2 seconds |
Noise level | <55db |
Grinding method | Wet grinding, dry grinding and low temperature grinding are all available |
Refrigeration | Has the ability to upgrade to ultra-low temperature liquid nitrogen refrigeration or air refrigerator refrigeration |
Adapter material: | PTFE or alloy steel |
Full protection: | Fastening device with automatic center positioning, safety lock when working, full protection |
Grinding kit material: | Hard rigid, PTFE (Teflon) zirconia |
Dimensions | 280*400*530 |
Weight | 23 KG |
Basic Configuration:
? One Tissuelyser? host | ? One set of 2ml adapter, one set of 2ml refrigeration adapter, one set of 1.5ml refrigeration fluid adapter (optional) |
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? 1000 imported stainless steel grinding beads on No. 5, 1000 imported ceramic grinding beads on No. 5 (optional), and imported stainless steel grinding beads on No. 3 | ? 200 special grinding tubes ? One set of special bead gun (optional) |
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Experimental case:
1. The experimental method of Jingxin plant tissue grinder to grind rice leaves | 2. High-throughput grinding of corn seeds to extract nucleic acid and protein |
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Remark：
* The supporting jingxin kit can be used to extract DNA/RNA proteins in samples quickly, efficiently, stably and in batches
* 1HZ=30 rev/sec 1HZ=100RPM
* Conventional adapters can be placed in the refrigerator to provide a cold environment for the samples while working. It can be equipped with an adapter with refrigeration, which can be immersed in liquid nitrogen for more than 30 minutes.
* The noise emission value for the working environment depends on the type of sample and the setting of the grinder. The parameters in the table are in no-load state
* A small amount of samples usually used for DNA and RNA separation can be prepared in disposable centrifuge tubes. Adapters made of polytetrafluoroethylene materials can be used to accommodate 5 or 10 disposable sample tubes for cell disruption. In multi-sample tissue The cell tissue in the grinder is quickly and effectively broken in a short time, so there is no need for additional low-temperature refrigeratio
Experimental case:
Extract objects | Jingxin supporting kit | |
Animal tissue | DNA | jingxin-1 |
RNA | jingxin-2 | |
Protein | jingxin-3 | |
Plant tissue | DNA | jingxin-4 |
RNA | jingxin-5 | |
Protein | jingxin-6 | |
microorganism | DNA | jingxin-7 |
RNA | jingxin-8 | |
Protein | jingxin-9 | |
